全文获取类型
收费全文 | 2207篇 |
免费 | 135篇 |
国内免费 | 1篇 |
出版年
2021年 | 19篇 |
2020年 | 8篇 |
2019年 | 18篇 |
2018年 | 20篇 |
2017年 | 26篇 |
2016年 | 30篇 |
2015年 | 46篇 |
2014年 | 54篇 |
2013年 | 130篇 |
2012年 | 122篇 |
2011年 | 126篇 |
2010年 | 60篇 |
2009年 | 62篇 |
2008年 | 104篇 |
2007年 | 108篇 |
2006年 | 103篇 |
2005年 | 105篇 |
2004年 | 87篇 |
2003年 | 125篇 |
2002年 | 94篇 |
2001年 | 84篇 |
2000年 | 85篇 |
1999年 | 76篇 |
1998年 | 27篇 |
1997年 | 29篇 |
1996年 | 28篇 |
1995年 | 28篇 |
1994年 | 21篇 |
1992年 | 47篇 |
1991年 | 33篇 |
1990年 | 37篇 |
1989年 | 42篇 |
1988年 | 30篇 |
1987年 | 36篇 |
1986年 | 42篇 |
1985年 | 31篇 |
1984年 | 30篇 |
1983年 | 22篇 |
1982年 | 25篇 |
1981年 | 18篇 |
1980年 | 8篇 |
1979年 | 15篇 |
1978年 | 9篇 |
1977年 | 10篇 |
1976年 | 7篇 |
1975年 | 11篇 |
1974年 | 9篇 |
1973年 | 10篇 |
1972年 | 6篇 |
1967年 | 8篇 |
排序方式: 共有2343条查询结果,搜索用时 187 毫秒
41.
Development of DNA markers for identifying chrysanthemum cultivars generated by ion-beam irradiation
Tsukasa Shirao Kei-ichiro Ueno Tomoko Abe Tomoki Matsuyama 《Molecular breeding : new strategies in plant improvement》2013,31(3):729-735
Four chrysanthemum cultivars were generated through (carbon) ion-beam irradiation of the original ‘Jimba’ (Chrysanthemum morifolium Ramat.). The new cultivars had acquired a number of superior cultivation traits, while remaining identical to the commercially available ‘Jimba’ in appearance. In this study, polymerase chain reaction (PCR) assays were used to detect the mutated region of each strain, thereby allowing clear identification at the molecular level. PCR assays were performed with 446 primer sets, including random amplified polymorphic DNA (RAPD) primer sets (10-mer RAPD), arbitrarily primed (AP)-PCR primers based on retrotransposon-like sequences and modified RAPD primers (15-mer RAPD). 15-mer RAPD primers generated a 1.49-fold increased band number at high annealing temperatures compared with the original 10-mer RAPD primers and could thus be effective for detection of polymorphic patterns. Our results provide information on the mutated regions of these ion-beam-irradiated chrysanthemum cultivars. Thus, specific DNA markers could be used to improve identification of new cultivars of chrysanthemum as well as other clonal cultivars of horticultural and agricultural crops. 相似文献
42.
Farsenyl pyrophosphate synthase is a potential molecular drug target of risedronate in Babesia bovis
Akio Ueno Mohamad Alaa Terkawi Miki Yokoyama Shinuo Cao Gabriel Aboge Mahmoud Aboulaila Yoshifumi Nishikawa Xuenan Xuan Naoaki Yokoyama Ikuo Igarashi 《Parasitology international》2013,62(2):189-192
A cDNA encoding farnesyl pyrophosphate synthase of Babesia bovis (BbFPPS) has been isolated, cloned and characterized as molecular drug target. Sequence analysis revealed that BbFPPS contains an open reading frame of 1011 bp with predicted 336 amino acids and molecular mass of 38 kDa. Antiserum raised in mice against recombinant BbFPPS expressed in Escherichia coli specifically reacted with native protein of B. bovis parasites by Western blot analysis and indirect immunofluorescent test. Enzymatic assay using recombinant BbFPPS revealed that the Km value of the enzyme for isopentenyl pyrophosphate and dimethylallyl pyrophosphate was 2.494 ± 1.536 μM. Risedronate inhibited the activity of BbFPPS yielding IC50 value of 8.4 ± 1.2 nM. Furthermore, the in vitro growth of B. bovis was significantly inhibited in the presence of a micromolar concentration of risedronate (IC50 = 4.02 ± 0.91 μM). No regrowth of B. bovis was observed at 10 μM of risedronate in the subsequent viability test. These results demonstrate that BbFPPS is the molecular target of risedronate, which could inhibit the in vitro growth of B. bovis. 相似文献
43.
Kenji Ishii Junko Adachi Masafumi Tomita Masahiro Kurosaka Yasuhiro Ueno 《Free radical research》2013,47(2):163-168
The aim of this study is to evaluate oxidative stress in man after paraquat ingestion by analyzing 7 f - and 7 g -hydroperoxycholest-5-en-3 g -ol (7 f - and 7 g -OOH) as well as oxysterols, cholesterol oxidation products, as indices of lipid peroxidation. Lung, kidney, and liver were collected at autopsy from seven patients with paraquat poisoning and seven controls matched for age and sex. We identified for the first time 7-ketocholesterol (7-keto) and 7-hydroxycholesterol (7 f -OH and 7 g -OH) in human kidney by LC-MS. Next, we quantified 7 f -OOH and 7 g -OOH by HPLC with postcolumn chemiluminescence as well as oxysterols by HPLC-UV. Both 7 f -OOH and 7 g -OOH detected in lung and kidney from the controls were as low as the paraquat group. In contrast, we found both 7-keto and 7 g -OH in lung and 7-keto in kidney from the paraquat group were significantly higher than from the controls. This is the first report on accumulated oxysterols in lung and kidney from human paraquat poisoning. It seems to reflect greater oxidative stress in the pathology of paraquat intoxication. 相似文献
44.
Kentaro Uchida Kouji Naruse Masashi Satoh Kenji Onuma Masaki Ueno Shotaro Takano Ken Urabe Masashi Takaso 《Experimental Animals》2013,62(3):255-265
Although recent studies suggest that hyperlipidemia is a risk factor for osteoarthritis
(OA), the link between OA and hyperlipidemia is not fully understood. As the number of
activated, circulating myeloid cells is increased during hyperlipidemia, we speculate that
myeloid cells contribute to the pathology of OA. Here, we characterized myeloid cells in
STR/Ort mice, a murine osteoarthritis model, under hyperlipidemic conditions. Ratios of
myeloid cells in bone marrow, the spleen, and peripheral blood were determined by flow
cytometry. To examine the influence of the hematopoietic environment, including abnormal
stem cells, on the hematopoietic profile of STR/Ort mice, bone marrow transplantations
were performed. The relationship between hyperlipidemia and abnormal hematopoiesis was
examined by evaluating biochemical parameters and spleen weight of F2 animals
(STR/Ort x C57BL/6J). In STR/Ort mice, the ratio of CD11b+Gr1+ cells
in spleens and peripheral blood was increased, and CD11b+Gr1+ cells
were also present in synovial tissue. Splenomegaly was observed and correlated with the
ratio of CD11b+Gr1+ cells. When bone marrow from GFP-expressing mice
was transplanted into STR/Ort mice, no difference in the percentage of
CD11b+Gr1+ cells was observed between transplanted and age-matched
STR/Ort mice. Analysis of biochemical parameters in F2 mice showed that spleen
weight correlated with serum total cholesterol. These results suggest that the increase in
circulating and splenic CD11b+Gr1+ cells in STR/Ort mice originates
from hypercholesterolemia. Further investigation of the function of
CD11b+Gr1+ cells in synovial tissue may reveal the pathology of OA
in STR/Ort mice. 相似文献
45.
Yoshihito Ueno Shinji Ishihara Yasutomo Ito Yukio Kitade 《Nucleosides, nucleotides & nucleic acids》2013,32(4-6):475-487
The synthesis and properties of oligonucleotides (ONs) containing 9-(2,3,4-trihydroxybutyl)adenine, A C2 and A C3, are described. The ON containing A C2 involves the 3′ → 4′ and 3′ → 5′ phosphodiester linkages in the strand, whereas that containing A C3 possesses the 3′ → 4′ and 2′ → 5′ phosphodiester linkages. It was found that incorporation of the analogs, A C2 or A C3, into ONs significantly reduces the thermal and thermodynamic stabilities of the ON/DNA duplexes, but does not largely decrease the thermal and thermodynamic stabilities of the ON/RNA duplexes as compared with the case of the ON/DNA duplexes. It was revealed that the base recognition ability of A C2 is greater than that of A C3 in the ON/RNA duplexes. 相似文献
46.
Akash Chandela Taeko Watanabe Kenji Yamagishi Yoshihito Ueno 《Bioorganic & medicinal chemistry》2019,27(7):1341-1349
With the aim to create a small interfering RNA (siRNA) with enhanced activity and resistance to nuclease degradation, we synthesized and evaluated the properties of the following siRNAs containing haloalkyl β-d-ribofuranosides at their 3′-dangling ends: 2,2,2-trifluoroethyl β-d-ribofuranoside, 2,2,2-trichloroethyl β-d-ribofuranoside and 2,2,2-tribromoethyl β-d-ribofuranoside. The gene silencing activities of the modified siRNAs were investigated through a dual luciferase reporter assay using HeLa cells. The highest silencing activity was observed for the trichloroethyl analog modified siRNA, which was closely followed by the trifluoroethyl and tribromoethyl analogs. The modified siRNAs were found to show increased binding affinity towards the Piwi-Argonaute-Zwille (PAZ) domain protein based on computational analysis and an experimental study. Furthermore, the RNAs modified with the analogs at their 3′-ends exhibited improved resistance to hydrolysis by a 3′-exonuclease. 相似文献
47.
Sakamoto Kazunori Ogiwara Natsuko Kaji Tomomitsu Sugimoto Yurie Ueno Mitsuru Sonoda Masatoshi Matsui Akihiro Ishida Junko Tanaka Maho Totoki Yasushi Shinozaki Kazuo Seki Motoaki 《Journal of plant research》2019,132(4):541-568
Journal of Plant Research - Soybean (Glycine max) roots establish associations with nodule-inducing rhizobia and arbuscular mycorrhizal (AM) fungi. Both rhizobia and AM fungi have been shown to... 相似文献
48.
Nagao Ryo Ueno Yoshifumi Akita Fusamichi Suzuki Takehiro Dohmae Naoshi Akimoto Seiji Shen Jian-Ren 《Photosynthesis research》2019,140(2):141-149
Photosynthesis Research - Diatoms are dominant phytoplankton in aquatic environments and have unique light-harvesting apparatus, fucoxanthin chlorophyll a/c-binding protein (FCP). Diatom... 相似文献
49.
50.
Akari Nitta Kazuya Hori Isei Tanida Ayumi Igarashi Yasuyo Deyama Takashi Ueno Eiki Kominami Manabu Sugai Koji Aoki 《Biochemical and biophysical research communications》2019,508(2):521-526
Autophagy, a system for the bulk degradation of intracellular components, is essential for homeostasis and the healthy physiology and development of cells and tissues. Its deregulation is associated with human disease. Thus, methods to modulate autophagic activity are critical for analysis of its role in mammalian cells and tissues. Here we report a method to inhibit autophagy using a mutant variant of the protein ATG7, a ubiquitin E1-like enzyme essential for autophagosome formation. During autophagy, ATG7 activates the conjugation of LC3 (ATG8) with phosphatidylethanolamine (PE) and ATG12 with ATG5. Human ATG7 interactions with LC3 or ATG12 require a thioester bond involving the ATG7 cysteine residue at position 572. We generated TetOff cells expressing mutant ATG7 protein carrying a serine substitution of this critical cysteine residue (ATG7C572S). Because ATG7C572S forms stable intermediate complexes with LC3 or ATG12, its expression resulted in a strong blockage of the ATG-conjugation system and suppression of autophagosome formation. Consequently, ATG7C572S mutant protein can be used as an inhibitor of autophagy. 相似文献